Abstract
Angiotensin converting enzyme (ACE) was immobilized on chitosan microspheres with glutaraldehyde as cross-linking reagent. The activity and stability of immobilized ACE (I-ACE) were improved by further modification with alkylamines. The modification conditions were optimized. The characteristics of I-ACE and modified I-ACE (MI-ACE) including activity, kinetic parameters, optimal pH and temperature, stability and reusability were investigated. After modification, I-ACE activity was increased, Km decreased from 4.33 mM to 2.61 mM, optimal pH value was altered, pH stability, storage stability and thermal stability were improved, and optimal temperature did not change.
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