Abstract
Among the most studied natural polymers, alginate, a natural polysaccharide extracted from algae is well known due to its applications related to remarkable properties such as biocompatibility, biodegradability and low toxicity. In order to optimize the physicochemical properties of alginate and starch-based biofilms reinforced by glycerol, we propose of different biofilms by Raman spectroscopy according to the mass proportion of alginate in water: 1%, 2%, 3%, 4% and 5%. The first vibrational study of alginate-based biofilms demonstrated a link between the mass proportion of alginate in water with the contribution of hydrogen bonds through vibrational modes at 1570, 1500 and 1414 cm−1 and with the vibrational contributions of gauche and trans conformational C-C stretching at 1098 and 1068 cm−1, respectively. This link seems to persist in the case of biofilms based on alginate reinforced by glycerol with a lower intensity. For the cases of alginate and starch-based biofilms and glycerol-reinforced alginate–starch biofilms, the impact of the mass proportion of alginate in water on the hydrogen bonds is evaluated by determining an area ratio τ=Area1414/Area1340 between the mode 1414 cm−1 assigned to the hydrogen bonds and 1340 cm−1 assigned to d(O-H) in plane. The vibrational results show interaction between the proportion of alginate and the hydrogen bonds.
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