Abstract
The subcellular distribution and accumulation of LHRH in hypothalamic neurons of female rats were studied as a function of age (1-24 months). Hypothalami were homogenized in isoosmotic sucrose solution, and a 900 × g supernatant fluid was prepared. Free granules and synaptosomes containing LHRH were separated by means of continuous sucrose density gradient centrifugation. Homogenization and subcellular fractionation were carried out at 4 or 25 C. In the homogenates, LHRH was found to be associated with free granules and with two types of synaptosome-like particles: rigid synaptosomes (the presence of which was evident after homogenization and fractionation were carried out at 4 as well as 25 C) and fragile synaptosomes (the presence of which was evident only after homogenization and fractionation at 25 C). The amount of LHRH recovered in rigid synaptosomes prepared from hypothalami of 1.5-month-old rats was significantly (P < 0.01) greater than that in rigid synaptosomes prepared from 1-month-old animals....
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