Abstract
The activities of adenyl cyclase and phosphodiesterase were studied in epididymal fat cells isolated from young (5–6 week), middle-aged (10–12 week) and old (18–24 weeks) rats. Adenyl cyclase activity in fat cell homogenates was assayed by the formation of adenosine 3′,5′-monophosphate (cyclic 3′,5′-AMP) from ATP. The NaF-and norepinephrine-stimulated adenyl cyclase activity was much lower in fat cell homogenates of old rats than in those of the younger rats. Adenyl cyclase activity in intact fat cells was assayed by measuring the accumulatiion of 3H-labeled cyclic 3′,5′-AMP by cells which had been previously labeled with [ 3H]adenine. The norepinephrine stimulated adenyl cyclase activity in intact fat cells also decreased markedly in older rats. By contrast, phosphodiesterase activity was higher in fat cells of the older rats.
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