Effect of aging on brain volume and cognitive function in Sprague-Dawley rats

Abstract

Event Abstract Back to Event Effect of aging on brain volume and cognitive function in Sprague-Dawley rats Hamizah S. Hamezah1, 2, Lina W. Durani1, 2, Nor F. Ibrahim1, 2, Daijiro Yanagisawa1, Tomoko Kato1, Akihiko Shiino1, Hanafi A. Damanhuri2, Wan Z. Wan Ngah2 and Ikuo Tooyama1* 1 Shiga University of Medical Science, Molecular Neuroscience Research Center, Japan 2 Universiti Kebangsaan Malaysia, Department of Biochemistry, Faculty of Medicine, UKMMC, Malaysia Aging is associated with impairments in cognitive functions and locomotor activity, as well as alterations in the brain volume. Therefore, knowing the time of onset at which these alterations occur during aging may help us understanding mechanisms leading to the aging brain. This study was conducted to determine cognitive and brain volume changes of middle to late aged stages of aging in animal model. Forty-four male Sprague-Dawley rats were divided into four groups: 14, 18, 23, and 27 months old. 1H MRI was performed on the rats, using a 7.0-Tesla MR scanner system. Images of medial prefrontal cortex (mPFC), hippocampus, striatum, lateral ventricle, and cerebellum were analyzed using ImageJ software. Open field, object recognition, and Morris water maze were conducted after MRI measurement to assess locomotor and cognitive functions. The volume of mPFC, hippocampus, striatum, lateral ventricle, and cerebellum were then measured. Our results showed that the specific brain volume did not significantly change with age. Open field test indicated significant decline in the number of line crossing and central entries in 27 months old rats compared with 14 months, which represented locomotor activity deficits at the late-aged rats. Furthermore, impairments in memory of the 27 months old rats were also shown by the significantly lower recognition index to the displaced objects compared with 14 months. Morris water maze results showed that 27 months old rats took significantly longer time and distance to reach the hidden platform compared with 14 months. Probe test indicated significantly lower number of platform crossings in the 27 months old rats compared with 14 months. In conclusion, age-related locomotor and cognitive deficits occur during aging, with most marked impairments observed at late-aged stage. However, no brain volume changes observed across the different ages groups. Keywords: Aging, in vivo, Cognitive Function, magnetic resonance imaging (MRI), brain volume Conference: 14th Meeting of the Asian-Pacific Society for Neurochemistry, Kuala Lumpur, Malaysia, 27 Aug - 30 Aug, 2016. Presentation Type: Poster Presentation Session Topic: 14th Meeting of the Asian-Pacific Society for Neurochemistry Citation: Hamezah HS, Durani LW, Ibrahim NF, Yanagisawa D, Kato T, Shiino A, Damanhuri HA, Wan Ngah WZ and Tooyama I (2016). Effect of aging on brain volume and cognitive function in Sprague-Dawley rats. Conference Abstract: 14th Meeting of the Asian-Pacific Society for Neurochemistry. doi: 10.3389/conf.fncel.2016.36.00140 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 04 Aug 2016; Published Online: 11 Aug 2016. * Correspondence: Prof. Ikuo Tooyama, Shiga University of Medical Science, Molecular Neuroscience Research Center, Otsu, Shiga, Japan, kinchan@belle.shiga-med.ac.jp Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Hamizah S Hamezah Lina W Durani Nor F Ibrahim Daijiro Yanagisawa Tomoko Kato Akihiko Shiino Hanafi A Damanhuri Wan Z Wan Ngah Ikuo Tooyama Google Hamizah S Hamezah Lina W Durani Nor F Ibrahim Daijiro Yanagisawa Tomoko Kato Akihiko Shiino Hanafi A Damanhuri Wan Z Wan Ngah Ikuo Tooyama Google Scholar Hamizah S Hamezah Lina W Durani Nor F Ibrahim Daijiro Yanagisawa Tomoko Kato Akihiko Shiino Hanafi A Damanhuri Wan Z Wan Ngah Ikuo Tooyama PubMed Hamizah S Hamezah Lina W Durani Nor F Ibrahim Daijiro Yanagisawa Tomoko Kato Akihiko Shiino Hanafi A Damanhuri Wan Z Wan Ngah Ikuo Tooyama Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.