Effect of agents known to alter carbon tetrachloride hepatotoxicity and cytochrome P-450 levels on carbon tetrachloride-stimulated lipid peroxidation and ethane expiration in the intact rat

Abstract

Administration of carbon tetrachloride (CCl 4) to rats led to an increase in expired ethane by 15 min. Prior treatment of rats with phenobarbital led to a significant increase in both CCl 4-stimulated ethane expiration and hepatic microsomal lipid diene conjugation, while prior treatment with 3-methylcholanthrene or CCl 4 led to a decrease in both parameters. Treatment with isopropanol increased CQ 4-stimulated ethane expiration, while neither ethanol nor diethyl maleate treatment altered the response to CCl 4. Cobaltous chloride treatment significantly decreased CCl 4-stimulated ethane expiration. A strong correlation was found between CCl 4-stimulated hepatic microsomal lipid diene conjugation and ethane expiration.