Abstract
Advanced Glycation End Products (AGE) are proteins that can cause cell destruction by increasing oxidative stress and inflammation. This product resulted from a series of chemical reactions after an initial glycation reaction. A Leydig cell is one of the types of cells affected by AGE. This cell is located in the interstitial of the testes and stimulated by the luteinizing hormone. This study aimed to compare the luteinizing hormone receptor levels in Leydig cell culture of Sprague-Dawley rat induced by AGE only and the one that administered gamma-mangosteen. We conducted an experimental laboratory study on luteinizing hormone receptor levels in Leydig cell culture of Sprague-Dawley rats induced by advanced glycation end products 200 μg/mL and given gamma-mangostin 5 μM compared to the one that was not given gamma-mangostin. The highest mean of LHR level was in group 3 given gamma-mangostin 5 μM (8.06 pg/ cells/24h), and the lowest mean was in group 1 (control) (7.78 pg/ cells/24h). The LHR levels in the rats' Leydig cell culture given 5μM gamma-mangostin were higher than the other groups indicate the inhibition capacity on the oxidation process caused by AGE in aging rats Leydig cells culture.
Highlights
Infertility, as a major health problem, occurs in an average of 15% –20% of couples
With the increase of male infertility cases caused by hormonal problems, the authors are interested in examining the luteinizing hormone receptors levels in Leydig cell cultures of Sprague-Dawley rats induced by Advanced glycation end-products (AGE) (200 μg/mL) and treated with gamma-mangostin (5 μM)
This result may be indicating that gamma-mangostin can inhibit the oxidation process caused by AGE so that luteinizing hormone receptors (LHR) remains expressed in Leydig cell culture
Summary
Infertility, as a major health problem, occurs in an average of 15% –20% of couples Both male and female factors can be the cause of infertility. Sperm production depends on adequate testosterone levels maintained by Leydig cells in response to Luteinizing Hormone which is secreted by the anterior pituitary. This hormone acts on the surface of testicular Leydig cells by binding with luteinizing hormone receptors (LHR)(Goluža et al, 2014; Zirkin & Papadopoulos, 2018). With the increase of male infertility cases caused by hormonal problems, the authors are interested in examining the luteinizing hormone receptors levels in Leydig cell cultures of Sprague-Dawley rats induced by AGE (200 μg/mL) and treated with gamma-mangostin (5 μM)
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