Abstract
Objective: To determine the effect of age and frequency of collection on quality parameters of Jersey bull semen at National Livestock Breeding Center (NLBC), Pokhara.
 Materials and Methodology: Nine Jersey bulls were selected randomly from NLBC. To obtain the effect of age, six bulls of three different age interval (3-4, 5-7 and 8-9 years) were selected randomly. Remaining three bulls were used to determine the effect of frequency of collection on semen quality. Studies were conducted for three months (Nov/Dec to Dec/Jan, 2016). Semen quality was analyzed using CASA system and SMILE software. ANOVA and paired t- tests were applied to find out level of significance. All the statistical analyses were done using SPSS 17.0
 Results: Sperm concentration, post- thaw motility, live sperm, and abnormal sperm were significantly affected by the age of the bulls. Semen quality was found superior in the bulls belonging to 5-7 years of age intervals. Significantly higher value for semen volume, sperm concentration and post- thaw motility was obtained on second collection (P<0.05). However, volume initial motility, pre-filling motility, live sperm and abnormal sperm percentage did not differ significantly among different age intervals and between the frequencies of collection.
 Conclusion: Different quality parameters of semen like volume, concentration, initial motility, pre-filling motility, post thaw motility, live and normal spermatozoa percentages were higher in 5-7 years old bulls and in second collection of semen.
 Int. J. Appl. Sci. Biotechnol. Vol 7(1): 88-95
Highlights
Livestock plays a vital role to the Nepalese economy and contributes about 11% to the total national gross domestic product (GDP) and about 26.8% to national agricultural gross domestic products (AGDP) (MoAD, 2016)
Sperm concentration, post- thaw motility, live sperm, and abnormal sperm were significantly affected by the age of the bulls
Different quality parameters of semen like volume, concentration, initial motility, pre-filling motility, post thaw motility, live and normal spermatozoa percentages were higher in 5-7 years old bulls and in second collection of semen
Summary
Livestock plays a vital role to the Nepalese economy and contributes about 11% to the total national gross domestic product (GDP) and about 26.8% to national agricultural gross domestic products (AGDP) (MoAD, 2016). Dairying is the most important sub-sector contributing about 63% to the total Livestock Gross Domestic Products (LGDP) which is about 8% of total national GDP. It provides a daily source of income and has been well recognized as a means for poverty reduction across the developing world. In Nepal, the demand for milk and milk products is increasing. There is a need of high yielding breeds of dairy animals in order to meet the increased demand of milk.
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