Effect of aerobic microbes' competition for oxygen on nitrogen removal in mainstream nitritation-anammox systems.

Abstract

The effects of C/N ratio in mainstream partial nitritation (PN)-anaerobic ammonia oxidation (ANAMMOX) considering competitive relationship of aerobic microbes competing for oxygen were investigated. Thy system was operated for 501 d with various C/N ratio. Competitive growth of aerobic heterotrophic bacteria (AHB) at ≥ 1 of C/N ratio acted effectively on the selective inhibition of nitrite-oxidizing bacteria (NOB) while contributing to stable PN-A. In-depth kinetic analysis indicated oxygen affinity of aerobic microbes was in the order of AHB > ammonia-oxidizing bacteria (AOB) > NOB. In addition, potential of denitritation by AHB could contributed to improving nitrogen removal up to 87.5 ± 4.3%. AHB was comparatively clustered into two groups with a C/N ratio of 1. Nitrosomonas sp. PY1 became predominant while Nitrospira spp. were the major NOB. The potential of AHB in establishing selective inhibition of NOB was identified, which could be a novel approach to stabilze the mainstream PN-A.