Effect Of Aerobic Capacity, Ampk-pgc-1α Pathway In Skeletal Muscle For Detraining Of Different Training

Abstract

PURPOSE: By observing HIIT group rats and MIT group rats’ changes in the timing and content of AMPK and PGC-1α in skeletal muscle, changes in aerobic capacity given the same load and training time and then stop the training. To provide a reasonable arrangement of retraining programs. METHODS: 27 4-month-old male Wistar rats were randomly divided into 3 groups (N=9): control (Group C), MIT (Group M), HIIT (Group H). C do not exercise, H training at 50%-70%-90% VO2max intensity according to the VO2max test results, M training at 70% VO2max intensity. Both H and M were performed for 50min/day, 5 days/week for 6 weeks of training. Rats were randomly selected from 3 groups on the 1st, 3rd, 7th, and 10th day after the training was stopped. They were intraperitoneal injected by 5% chloral hydrate to get soleus muscle. Using western blot to analysis AMPK and PGC-1α. The results are expressed as mean±standard deviation. one-way ANOVA and non-parametric test are performed in accordance with the normal distribution. RESULTS: 1.The AMPK in the skeletal muscle in H was significantly higher than that in C (P<0.05). The trend of AMPK in M was similar to that in H. 2.The level of PGC-1α in skeletal muscle compared to C was significantly increased in H(P<0.05). The change trend of PGC-1α in M was similar to that in H. 3.The VO2max showes a downward trend. CONCLUSIONS: 1.After 6 weeks of HIIT or MIT, all indicators showed a downward trend. 2.HIIT is superior to MIT in improving and maintaining AMPK, and PGC-1α level ,but it is less effective in maintaining VO2max.