Abstract
The growth kinetics of Thermus thermophilus HB27 was investigated in rich medium (Thermus medium) under batch cultivation at 65°C in 3-l fermentors. The growth and oxygen consumption rates were highly dependent on the aeration and agitation rates. Volumetric mass transfer coefficient (KLa, h−1) and hence oxygen transfer rate (OTR, mol m −3 h−1) into the fermentation broth increased with increased aeration and/or agitation rates. A KLa and OTR of 175.4 h−1 and 31.7 mol m−3h−1 respectively, corresponding to 500 rpm agitation and2 vvm aeration with a mixture of air and oxygen, were required to avoid oxygen limitation. The maximum growth rate (μmax, h−1), doubling time (tD, h), and dry cell weight determined for T. thermophilus HB27 growing under these conditions were 0.27 h−1, 2.67 h, and 3g/l respectively. This cell yield is higher than any previously published reports for growth of Thermus cultures, including studies that employed pressurized bioreactors.
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