Effect of Advanced Glycosylation End Products on the Induction of Nitric Oxide Synthase in Murine Macrophages

Abstract

We studied the role of advanced glycosylation end products on the induction of nitric oxide synthase in peritoneal mouse macrophages previously exposed to modified BSA. A dose-dependent increment in the nitric oxide production induced by LPS and IFN-γ was observed when cell cultures were pretreated with modifed BSA for 48 hours. In addition, the up regulation of nitric oxide production was also time-dependent, being maximal at 24–48 hours. Experiments carried out in the presence of neutralizing antibodies to IL-1 and TNF-α, suggested that up regulation was not due to the capacity of modified BSA to induce both proinflammatory signals. The up regulation of nitric oxide production was paralelled with an increase in iNOS mRNA.