Effect of adsorbed protein on the adhesion behaviour of platelet to the microdomain surface of 2-hydroxyethyl methacrylate-styrene block copolymer

Abstract

In order to design antithrombogenic polymers, adhesivity and shape change on platelets on the block copolymer composed of 2-hydroxyethyl methacrylate (HEMA)-styrene were investigated with respect to the influence of an adsorbed layer of plasma proteins. When the polymer surface was pre-adsorbed with serum albumin, the amount and deformation of adhered platelets were decreased on block copolymer and homopolymer surfaces. Pre-adsorption of γ-globulin on poly(HEMA) surface caused significant shape change of platelets, which was considered to be due to the binding of Fc part of γ-globulin and Fc receptor of platelet membrane. On the block copolymer surface, shape change and aggregation of platelets were effectively suppressed with and without protein pre-adsorption in comparison with the corresponding homopolymers of poly(HEMA) and polystyrene. It was found that the microdomain structure surface of the block copolymer showed an activity to suppress the shape change of adhered platelets both with and without protein pre-adsorption.