Abstract

The pathogenicity of the fungus, Metarhizium anisopliae, commonly referred to as the green muscardine fungus, has been demonstrated against numerous insect species inhabiting various habitats. Laboratory experiments were carried out to investigate the impact of naturally derived adjuvants on the growth and spore characteristics of M. anisopliae. The natural polysaccharide (guar gum, gum acacia and carboxymethyl cellulose), vegetable oil (groundnut and cottonseed oil) and a synthetic adjuvant were used in the study at three distinct concentrations: 0.05%, 0.1%, and 0.2% v/v or w/v. Among the various adjuvants tested, both gum acacia and guar gum at concentrations of 0.1% and 0.2% were observed to have a positive impact on the growth of the fungus, resulting in enhanced radial mycelial growth, conidiospore production, surface area coverage, and conidiospore germination. Conversely, this fundamental study highlights the detrimental effects of synthetic adjuvants on the growth and spore characteristics of M. anisopliae.

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