Effect of adipose-derived stem cell conditioned medium on UVB damage of human dermal fibroblasts in different senescent degrees

Abstract

Objective To study the differences of UVB damage among yong, intermediate and aged human dermal fibroblasts (HDFs), the recovery effect of adipose-derived stem cell conditioned medium (ADSC-CM) on UVB damage and the relationship between the recovery effect and ADSC-CM concentration as well as its treating period. Methods HDFs were cultured at different senescent degrees in vitro to establish UVB damage model of HDFs, and then the HDFs were cultured in different concentration ADSC-CM and cellular proliferation was detected. Results The optimal treating period and concentration of ADSC-CM were 48h and 100% respectively. As to HDFs in different senescent degrees, ADSC-CM was able to accelerate cellular proliferation in both UVB group and control group. Conclusions Independently or simultaneously, the intrinsic and extrinsic factors can cause ageing damages on HDFs. To some extent, ADSC-CM has recovery effect on the damages through secretomes from ADSC. Key words: Adipose-derived stem cells conditioned medium; HDFs; Cellular senescence