Effect of adiponectin on the expression of PPARα,SREBP-1C, apoA5, and triglyceride in liver of diabetic rats

Abstract

Objective To observe the gene and protein expression of PPARα,sterol regulatory element binding proteins-1C (SREBP-1C),and apolipoprotein (apo) A5 under adiponectin intervention,as well as triglyceride level in liver in diabetic rats.Methods Sixty male Wistar rats were assigned into 3 groups in random:diabetic group(DM,n =20),adiponectin treated group (Ad-APN + DM,n =20),and control group (CON,n =20).10 rats from each group were taken to detect the gene and protein expression of PPARα,SREBP-1C,and apoA5 in liver by real time-fluorescent quantitation reverse transcription-polymerase chain reaction and Western blot.The level of triglyceride in liver was determined.Results (1) The gene and protein expressions of PPARα,apoA5 were decreased in DM group(vs CON,P＜0.05).The expression of SREBP-1C and the level of triglyceride were increased (P＜0.05).(2) The expression of PPARα and apoA5 in Ad-APN+DM group were increased while the expression of SREBP-1C was decreased(all P＜0.05).(3) No difference was found in the expressions of PPARα,SREBP-1C,and apoA5,as well as the levels of triglyceride between Ad-APN + DM group and CON group (P＞0.05).Conclusions Adiponectin may upregulate PPARα and apoA5 expressions but inhibit SREBP-1C expression in liver of diabetic rats,leading to decreased triglyceride content in liver of rats. Key words: Adiponectin; Diabetes mellitus, experimental; PPARα; Sterol regulatory element binding proteins-1C ; Apolipoprotein A5 ; Triglyceride