Effect of adenovirus- mediated interleukin- 6 gene on biological behavior of spinal cord astrocytes

Abstract

Objective To investigate the effect of interleukin- 6(IL- 6)gene on proliferation, migration and invasion activity of spinal cord astrocytes. Methods IL-6 gene was cloned into adenovirus vector, then the astrocytes particles expressing IL-6 were infected into astrocytes. Real- time quantitative polymerase chain reaction(Real- time PCR)and Western blotting were performed to examine the expression of IL-6 in infected astrocytes. The growth ability was detected by methyl thiazol tetrazolium(MTT) assay.The cell cycle was analyzed by flow cytometry. The adhension and migration were examined by matrix(MTX)and multi tetrazolium compounds(MTS)assays. Results Comparing to controls group and Ad- IL- 6- green fluorescent protein(GFP)group, the expression levels of IL- 6 mRNA and protein were both significantly up- regulated after 4 days of IL- 6 adenovirus infection. Comparing with the Ad- GFP group, MTT assay showed that the A490 of Ad- IL- 6- GFP group was up- regulated after adenovirus infection(P<0.05). Comparing with the Ad- GFP group, cell cycle test revealed IL- 6 gene could accelerate cells in G1[(5.40±0.90)%], and the proportion of KF cells was increased in S phase [(7.26±0.40)%]and G2 phase[(83.52±0.50)%]after cells were infected(P<0.01). Transfection of IL- 6 adenovirus stimulated the migration and invasion activity of astrocytes. Conclusion Adenovirus- mediated IL- 6 gene efficiently stimulate the proliferation, cell cycle progression, migration and invasion activity of astrocytes. Key words: Interleukin-6; Spinal cord; Astrocyte; Proliferation; Migration; Adhension