Abstract
When cationic polyelectrolytes such as diethylaminoethyl-dextran (DEAED) and poly(allylamine) were added to solutions of glucose dehydrogenase (GDH) from Bacillus megaterium IWG3, the thermal stability of GDH increased remarkably. With the addition of DEAED, the rate of GDH-catalyzed oxidation of β- d-glucose increased in the low concentration range of NAD +, and the Michaelis constant and inhibition constant for NAD + decreased. These results suggest that negatively charged GDH interacts with the cationic water-soluble polymers to form conjugates by electrostatic force, and also that negatively charged coenzymes are “adsorbed” by the polymers, resulting in enrichment of the coenzyme in the vicinity of GDH.
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