Effect of Added PPM on Murashige and Skoog Media for Shallot Meristematic Proliferation

Abstract

Shallot (Allium ascolonicum L) are plants that belong to genus Allium sp that were propagated vegetatively through bulbs. In the developed countries, shallot seed production can be produced through in vitro/micropropagation. The activity observed the effect of explant source and added of PPM ( Plant Preservative Mixture) on Murashige and Skoog media in the growth and development of explants (Shoot tip, meristematic). The activity has been conducted in the tissue culture laboratory of IVEGRI from January to April 2019. As an explant bulb of shallot cv. Bima Brebes were infected OYDV or SYSV that has been tested by serology DAS ELISA. Media composition was MS (1962) with supplement sucrose 30 g L-1+ IAA 2 mg L−1 + Kinetin 2 mg L−1 + GA3 0.01 mg L−1 + Myo inositol 100 mg L−1 + gel rite 2 g L−1, pH 5.7. The treatment were PPM concentration (0;0.25;0.50;0.75; 1 mL L−1) and the explant : shoot tip (A), meristematic (B). Results of the experiment on visual observation were carried out randomly on growth and development, serology test of OYDV, and SYSV of the plantlets. On visual observation obtained MS media composition with 1 ml L−1 PPM the percentage of proliferation, there were no different from other concentrations of PPM and proliferation ≥ 60% for all media composition. DAS ELISA test for plantlet infected OYDV and SYSV for both explants was 47.37% - 69.33%.