Effect of add different carbon and nitrogen source on the pullulan polysaccharide production from fungus Aureobasidium pullulans

Abstract

Ten local isolates of Aureobasidium pullulans were isolated from different plant leaves. The ability of these isolates for pullulans production using production medium was investigated. The highest production of biomass and pullulans were achieved from isolates (A5, A2)with (11.93, 23.5)g/l respactivily.
 The effect of eight carbon and eight nitrogen sources at final concentration (5%) on biomass and pullulan production from local isolates were tested. The results showed that glucose gave maximum production of polysaccharide from isolate (A7) with (15.5g/l).While the highest production of biomass were obtained when sucrose were used as a carbon source with (14.53)g/l from isolate (A5). Lactose and xylose failed to support polysaccharide and biomass production. Also Ammonium nitrate gave highest production of polysaccharide for all isolates tested were (22.9 g/l) from (A9). While the lowest production of polysaccharide and biomass were obtained when urea was used as a nitrogen source(1.15 ,0.28 g/l)from isolates (A6, A9) respectively .The highest production of biomass was obtained in case of using ammonium sulphat as a nitrogen source with (18.97 g/l) from isolate (A9).