Abstract
The effect of acute stress on the incorporation of serine-1-C14 into the anterior and posterior pituitary lobes and the hypothalamus was studied. Stress produced an increased uptake of the labeled amino acid into the glacial acetic acid extract of the adenohypophysis. Using 2-dimensional electrophoretic and chromatographic separation on paper sheets, radioactive extracts were characterized. Every extract demonstrated the presence of the same radioactive peptide (Spot 1). The amount of radioactive incorporation into this spot was increased by stress. Using biological assay methods, Spot 1 was identified as ACTH. The electrophoretic and chromatographic characteristics of rat ACTH were markedly different from those of porcine or ovine ACTH. It was concluded that the effect of acute stress was to cause an increased synthesis of ACTH in the adenohypophysis of rats.
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