Abstract

This study aims to analyze the acute effect of omega 3 supplementation on serum TNF-α levels, pain intensity, and muscle strength after high-intensity weight training. A total of 20 adult males with a BMI of 18.00-24.99 were enrolled in this study. Subjects were divided into 2 groups, namely (K1) with placebo and (K2) with omega 3 supplementation with a dose of 1000 mg (540 mg EPA and 360 mg DHA). The intervention was carried out 24 hours after high-intensity weight training. The data in this study were taken before and after the intervention. Measurement of serum TNF-α levels using a human ELISA kit, measurement of pain intensity using visual analog scale (VAS), measuring muscle strength using a leg dynamometer. The data analysis technique is used if the data is normally distributed, namely Paired t-test and Independent t-test, if the data has not normally distributed the analysis used is the Wilcoxon signed-rank test. Omega 3 supplementation significantly reduced serum TNF-α levels (p-0.035), significantly reduced pain levels (p-0.007), and did not significantly decrease the strength (p-0.100). Omega 3 supplementations can reduce serum TNF-α levels, pain intensity, and omega 3 supplementations can maintain muscle strength after high-intensity weight training as evidenced by the absence of a significant decrease in muscle strength after high-intensity weight training.

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