Abstract
Adult female rats were exposed for 8 h to graded carbon-disulfide (CS2) concentrations between 20 and 400 ppm. It was found that the lipid content of the hepatic microsomal fraction rose significantly due to an increase in phosphatidylethanolamine, phosphatidylcholine, phosphatidylinositol, phosphatidylserine, sphingomyeline, lysophosphatidylcholine, cholesterol, triglycerides, diglycerides, and free fatty acids. The alterations occurred quickly and were completely reversible after exposure. Since the microsomal cytochrome P-450 content, as well as the activity of the microsomal NADPH cytochrome c-reductase, remained within the normal range following identical CS2 exposures, it is assumed that the alteration in the lipid pattern of the endoplasmic reticulum is a causal factor in inhibition of the microsomal-oxidative drug metabolism, induced by identical inhalatory CS2 doses. It is suggested that the altered lipid pattern impairs the microsomal membranes, thus affecting electron transport and resulting in a dysfunction of the oxidation chain. A slight elevation of the microsomal total protein was observed in relation both to a rise of the microsomal RNA content and an enhanced incorporation of [2,4 -3H]-L-phenylalanine into the liver microsomes after identical CS2 exposures. It is considered that this finding might represent an unspecific stimulatory reaction.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
