Abstract
Objectives: By observing the effect of acupoint catgut embedding on the intestinal flora of ovariectomized osteoporotic rats, the correlation between the regulation of intestinal flora and the prevention and treatment of osteoporosis was discussed preliminarily.Methods: By observing the effect of acupoint catgut embedding on the intestinal flora of ovariectomized osteoporotic rats, the correlation between the regulation of intestinal flora and the prevention and treatment of osteoporosis was discussed preliminarily.Result:Compared with Group A and Group B, bone mineral density (BMD) of femur and tibia in Group C decreased significantly (p<0.05), indicating successful modeling. Compared with Group C, BMD of femur and tibia in Group D was significantly increased (p<0.05). Compared with Group A and Group B, content of serum estrogens, calcium, and PTHrP in Group C were significantly decreased (p<0.05 or p<0.01). Content of serum estrogen, calcium, and PTHrP in Group D were significantly higher than those in Group C (p<0.05 or p<0.01). Compared with Group A and Group B, the abundance of mollicutes, actinobacteria, acidobacteria, chloroflexi and fusobacteria in group C was significantly increased. The abundance of probiotics in Group D such as lactobacillales, (lactobacillaceae, lactobacillus), bacillales, and streptococcus was significantly higher than that in Group C (p<0.05). The content of SCFAs such as acetic acid, butyric acid, iso-valeric acid, valeric acid and caproic acid in Group D were significantly higher than those in Group C, with statistical significance (p<0.05).Conclusions: Acupoint catgut embedding can effectively improve BMD, increase content of estrogen, calcium, PTHrP and SCFAs. Furthermore, embedding can also improve the abundance of probiotics and reduce the abundance of pathogenic bacteria. The changes of intestinal flora were correlated with the changes of estrogen and calcium, which may be one of the mechanisms of acupoint catgut embedding in the prevention and treatment of osteoporosis.
Highlights
To evaluate the effect of acupoint embedding on intestinal flora in rats with ovariectomized osteoporosis
Acupoint catgut embedding can improve bone density and increase the estrogen level in rats with osteoporosis, which maybe attributed to regulating composition and abundance of intestinal flora, and increasing the content of short-chain fatty acids
Our experiment showed that the content of Short-chain fatty acids (SCFAs) increased significantly after acupoint catgut embedding therapy compared with the untreated group, which may be achieved by increasing the abundance of probiotics
Summary
To evaluate the effect of acupoint embedding on intestinal flora in rats with ovariectomized osteoporosis. Most patients have symptoms, such as general weakness, bone pain, and fracture proneness[2], which seriously affects patients’ quality of life. It belongs to the category of "Guwei" and "Gubi" in traditional Chinese medicine. The study of acupoint catgut embedding mechanism for this disease mainly focuses on the improvement of bone density, estrogen level, and biochemical indicators of bone metabolism[3,4,5]. We propose to preliminarily explore the mechanism of acupoint catgut embedding therapy on postmenopausal osteoporosis by observing the changes of intestinal flora in ovariectomized rats
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