Abstract
AbstractA study was made of the uptake of actinomycin D, 3H‐thymidine, and 3H‐uridine in the axial regions of 11‐ to 13‐somite chick embryos cultured in vitro. Actinomycin D was taken up into all regions of embryos, with significantly more actinomycin D per cell in the axial region, including the node and unsegmented axial mesoderm (the lower axis). Demonstrable effects on RNA synthesis were restricted to tissue in the lower axial region. However, inhibition of DNA synthesis was seen in tissues of the upper axis already segmented, as well as in the lower axis. The differential response of the embryonic axis to actinomycin D is considered to be based on a greater demand for cells in the unsegmented and nodal region. Actinomycin D inhibited RNA synthesis, thereby inhibited DNA synthesis and cell division, and reduced availability of cells for growth and development. The paucity of cells in the node and unsegmented axial mesoderm restricted the formation of new somites and the laying down of new nerve cord and notochordal tissue. The upper axial region was not permanently affected.
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