Abstract
In this paper, we studied the effects of physical factors, such as, acoustic pulses of laser-induced hydrodynamics (ALIH) and extremely-high frequencies (EHF) radiation, on the formation of heterotopic bone marrow organs. A suspension of precipitated bone marrow cells from CBA mice were exposed to ALIH pulses and EHF radiation separately and in their combination tissue engineering constructs, presenting gelatin sponges 2 by 2 by 2[Formula: see text]mm in size containing 107 nucleated bone marrow cells, were exposed to physical factors and were implanted under the renal capsules of syngeneic mice. The newly formed hematopoietic organs were examined in three and five months later after treatment. The five months old transplants were bigger in size than the three months old transplants. The number of hematopoietic cells in the rest of the groups increased during this period by a factor from 3 to 10, the increase being as high as 20-fold in the ALIH[Formula: see text]EHF group. Maximal concentration of multipotent stromal cells (MSCs) was in the EHF[Formula: see text]ALIH, and minimal concentration was in the ALIH[Formula: see text]EHF. The accumulation rate of bone capsule weight was highest for the transplants of EHF[Formula: see text]ALIH and ALIH-sponge groups during the first three months. These data showed that the combined impact of the EHF[Formula: see text]ALIH on MSCs is the most effective for the formation of bone marrow transplantation.
Highlights
Nowadays, cell technologies constitute one of the most rapidly developing areas of biology and medicine
A suspension of precipitated bone marrow cells from CBA mice were exposed to acoustic pulses of laser-induced hydrodynamic (ALIH) pulses and extremely-high frequency (EHF) radiation separately and in combination in di®erent sequences ALIHþEHF, EHFþALIH
Exposure to EHF radiation and acoustic pulses of ALIG on multipotent stromal cells (MSCs) of rabbit did not lead to increasing proliferative activity of cells in vitro
Summary
Cell technologies constitute one of the most rapidly developing areas of biology and medicine. The concentration of stromal precursor cells in the bone marrow is (1–5)Â104; and in a suspension of hematopoietic cells they are strongly disunited by cells of di®erent kinds. For this reason, to increase the quantity of the stromal cells prior to their transplantation to the recipient, they are cultivated in vitro. E®ective methods were worked out to isolate these cells from the marrow and cultivate them in vitro, which provided for the increase in their numbers by more than 105 times.[16,17] Stromal progenitor cells, as well as their descendants have an enormous proliferation potential.[18] By the 10th passage the numbers ofbroblasts in some strains increase up to (1.2–7.2)Â109 cells. The cells undergoing up to 31–34 doublings in the course of their evolution
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