Effect of acetazolamide on brain swelling and aquaporin‐4 expression in rats brain after ischemic stroke

Abstract

ObjectiveTo study the effect of acetazolamide(AZA) on cerebral edema and aquaporin‐ 4 (AQP4) expression after cerebral infarction in rats. To explore the relations between ischemic cerebral edema and AQP4 expression.Methods150 male SD rats were randomly divided into three groups: sham operation group, non‐intervention group, AZA treatment group. Each group was divided into five subgroups according to the time of cerebral ischemia (6h, 1d, 3d, 5d and 7d), and there were 10 rats in each subgroup . The rats model of cerebral infarction was adopted by means of bilateral carotid arteries ligation(2‐VO) method. The treatment group was given intraperitoneal injection of AZA, while the sham operation group and the non‐intervention group were injected the same amount of normal saline at the corresponding time point above. The cerebral edema was measured by dry‐wet weight method. The expression of AQP4 in cerebral infarction was detected by immunohistochemistry.ResultsThe brain water content(BWC) and the expression of AQP4 in non‐intervention group and AZA treatment group began to increase at 6h after cerebral infarction, it reached the peak at 3d, and then decreased gradually.(P<0.05). The BWC and the expression of AQP4 in sham operation group had not change significantly at any time point above (P>0.05). At different time points, the BWC and the expression of AQP4 in non‐intervention group and AZA treatment group was higher than that in sham operation group.(P<0.05). The BWC and the expression of AQP4 in AZA treatment group was lower than that in non‐intervention group.(P<0.05). The expression of AQP4 in sham operation group had not change significantly at any time point above (P>0.05).ConclusionsAcetazolamide can alleviate rats cerebral edema and the expression of AQP4 after rats cerebral infarction, and it may alleviate rats cerebral edema by reducing the expression of AQP4 in rats brain.Support or Funding InformationThis study was supported by the National Natural Science Foundation Youth Fund (30600637), China Postdoctral Foundation(2014M561207), the Shanxi Province Basic Research Program ( 2010021034‐4, 201601D011119), the Shanxi Scholarship Council of China (2011‐096 and Key Projects No.4), Department of Human Resources and Social Security of Shanxi province, The science and technology research projects of health department of Shanxi Province(2016D011119), The Teaching Reform Program of Shanxi Higher Education , The Education Reform Project of Postgraduate Students in Shanxi Province. The Project of “131” Leading Talents of Shanxi Higher Education, The Education Reform Project of Shanxi Medical University.This abstract is from the Experimental Biology 2019 Meeting. There is no full text article associated with this abstract published in The FASEB Journal.