Effect of Acetaminofen Versus Lornoxicam Admistration on Oxidative Stress in Rat Hepatic and Renal Tissues

Mustafa Sancar Atac,Berrin Isik,Mustafa Bilge,Dervis Yilmaz,Saban Cem Sezen,Mustafa Arslan,Mustafa Kavutcu,Faruk Metin Comu

doi:10.17546/msd.71259

Mustafa Sancar Atac, Berrin Isik + Show 6 more

Open Access

https://doi.org/10.17546/msd.71259

Copy DOI

Abstract

Background and aim : The aim of study was to assess the oxidative status in rat hepatic and renal tissues after intraperitoneal administration of acetaminophen (AP) versus lornoxicam (L). Materials and Methods : For this purpose 18 Wistar Albino rats were randomly divided into 3 groups; each group consists of 6 rats. Group Control (Group C) remain untreated, comprises healthy rats. Group AP received AP (100 mg kg -1 ) and Group L received L (1.3 mg kg -1 ) intraperitoneally. Oxidative status was evaluated by MDA, SOD, GST and CAT in hepatic and renal tissues. Furthermore histopathalogical evaluation was performed in both tissues. Results : The lornoxicam received rats (Group L) showed significantly increased level of MDA and GST [(p=0.015), (p=0.048) respectively],decreased level of SOD (p=0.02)in liver tissue. Renal tissue MDA, SOD and GST activity and CAT levels were similar in groups [(p=0.168), (p=0.270), (p=0.686) respectively]. Histopathalogically Group AP and Group L more damaged than in Group C. Hepatic injury was moderate level in two groups. Minimal injury was observed in group AP. Renal injury in group L more than the Group AP. Conclusion : The results suggest that hepatotoxic effects of lornoxicam more than the AP while no remarkable difference nephrotoxicity.

Highlights

Nonsteroidal anti-inflammatory drug (NSAID)’s and acetaminophen (AP) have been used as an analgesic [1,2]
AP toxicity is dependent on the bioactivation by CYT enzymes to N-acetyl pbenzoquinoneimine (NAPQI) depletion of GSH, adduct formation to target proteins and oxidative stress
The purpose of this study was to determine the effects of intraperitoneally administered single dose AP versus L on oxidative stress in rat renal and hepatic tissue in terms of MDA, SOD, GST activity, CAT levels and tissue histopathalogy

Summary

Introduction

Nonsteroidal anti-inflammatory drug (NSAID)’s and acetaminophen (AP) have been used as an analgesic [1,2]. In spite of no toxicity is observed with therapeutic doses of AP both clinical and experimental studies revealed that even much lower doses can produce renal damage [2]. The aim of study was to assess the oxidative status in rat hepatic and renal tissues after intraperitoneal administration of acetaminophen (AP) versus lornoxicam (L). Oxidative status was evaluated by MDA, SOD, GST and CAT in hepatic and renal tissues. Results: The lornoxicam received rats (Group L) showed significantly increased level of MDA and GST [(p=0.015), (p=0.048) respectively],decreased level of SOD (p=0.02)in liver tissue. Renal tissue MDA, SOD and GST activity and CAT levels were similar in groups [(p=0.168), (p=0.270), (p=0.686) respectively]. Hepatic injury was moderate level in two groups. Renal injury in group L more than the Group AP. Conclusion: The results suggest that hepatotoxic effects of lornoxicam more than the AP while no remarkable difference nephrotoxicity

Objectives

Methods

Results

Discussion

Conclusion