Effect of Acellular Bovine Pericardium and Urinary Bladder Submucosa Matrixes in Reconstruction of Ventro-Lateral Hernias in Bucks; Molecular Evaluation

Areeg K M Al-Ebadi

doi:10.30539/iraqijvm.v43i1.474

Abstract

The present study aimed to estimate the efficiency of both a cellular bovine pericardium and bovine urinary bladder matrix sheets in the reconstruction of large ventro-lateral hernias in Iraqi bucks by using of molecular evaluation depending on real time-polymerase chain reaction technique to investigate the level of basic-fibroblast growth factor and vascular endothelial growth factor genes during the healing process and reconstruction of the abdominal defects. Under sedation and local anesthesia, (6cm X 8cm size) of ventro-lateral hernias were induced in 24 of Iraqi bucks. The animals were divided randomly into two main equal groups. In bovine pericardium-treatment group, the hernias were treated with onlay implantation of bovine pericardium. While, the hernias in UBM-treatment group were treated with onlay implantation of urinary bladder matrix, 30 days post-inducing of hernias. The molecular evaluation along the period of following-up recorded a significant up-regulation of the level of basic-fibroblast growth factor gene specific for presence of fibroblasts, myofibroblasts and collagen deposition in urinary bladder matrix -treatment group in comparison to bovine pericardium -treatment group with significant difference even at the end of the study. While, a significant up regulation of the levels of angiogenesis classic gene vascular endothelial growth factor were recorded in the bucks of bovine pericardium -treatment group compared to urinary bladder matrix -treatment group. In conclusion; molecular detection of the level of growth factors in target tissue can be used as an important criterion.

Highlights

Introduction prosthetic meshes has significantly diminishAbdominal wall defects correspond a difficult trouble, is a common acquired condition in ruminant's which has some harmless effects, such as lowering the productivity and reproductively of there (1and 2)
Surgical intrusion is a practical in these cases but wide ranging loss of abdominal wall may need a hernioplasty by provide biosynthetic mesh (Mesh repairs) by which angiogenesis and deposit new collagen formation produce by fibroblasts, as well as, decrease the strain that must be place on the abdominal wall in order to coat the hernia and are considered favorable for treatment of incisional hernias in general (3 and 4)
With the advance in clinical and sciences researches, biomaterial was revealed a material that interacts with biological systems for therapeutic purposes (8), these biomaterials scaffolds which are derived from different mammalian tissues, such as porcine small intestinal submucosa, acellular dermal matrix, urinary bladder matrix, human dura mater and pericardium have been employed to repair of musculotendinous, dermal, cardiovascular, gastrointestinal, lower urinary tract structures, esophageal, myocardial, musculoskeletal, as well as, the abdominal wall defect repairs (9 and 10)

Summary

Introduction

Introduction prosthetic meshes has significantly diminishAbdominal wall defects (hernia) correspond a difficult trouble, is a common acquired condition in ruminant's which has some harmless effects, such as lowering the productivity and reproductively of there (1and 2). The detection of the rate of hernia recurrence, these implants are non-absorbable and can cause infection, chronic pain and hernia recurrence to the surgical area which can lead to more complex operations (6). They may take part to the malfunction of other organs, such as the adherence of intestine, obstruction and creation of fistula (7). With the advance in clinical and sciences researches, biomaterial was revealed a material that interacts with biological systems for therapeutic purposes (8), these biomaterials scaffolds which are derived from different mammalian tissues, such as porcine small intestinal submucosa, acellular dermal matrix, urinary bladder matrix, human dura mater and pericardium have been employed to repair of musculotendinous, dermal, cardiovascular, gastrointestinal, lower urinary tract structures, esophageal, myocardial, musculoskeletal, as well as, the abdominal wall defect repairs (9 and 10). Preclinical and clinical evidences reveal that not all biologically derived scaffolds are the same and that host responses in tissue regeneration contrast among scaffold products (11)

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Results

Conclusion