Abstract
In carp acclimated to 20°C or 10°C intraerythrotic pH (pHi) and plasma pH (pHe) were determined in vitro after equilibration with CO 2 in either O 2 or N 2. ATP and GTP were determined with an enzymatic assay described in detail. The relationship between pHi, pHe and oxygen saturation was not affected by the acclimation temperature and was. (pHi – 6.10) = 0.853 – 0.159·S) · (pHe –6.21) There was a slight but significant decrease in ATP at 20°C. Apparent buffer values were affected by oxygenation and temperature. It is concluded from the recalculated CO 2 Bohr factor and from the temperature effect on the buffer value that carp hemoglobin forms carbamate which decreases at a higher temperature. These changes in ATP and carbamate can partly account for the increase in whole blood oxygen affinity in carp acclimated at a high temperature.
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