Abstract
Four kinds of endogenous metabolites, including creatinine, phenylacetylglycine, hippuric acid, and gluconic acid, were as potential biomarkers by a urinary metabolomics method in our previous study. To confirm them and investigate the effect of acarbose on type 2 diabetes in rats, a sensitive and selective ultra performance liquid chromatography tandem mass spectrometry (UPLC–MS/MS) method was developed to determine the concentration of the four kinds of endogenous metabolites in the urine of diabetes rats between pre- and post-treatment by acarbose. The results showed that the four kinds of endogenous metabolites were authenticated as the urinary biomarker of the diabetes rats. But after treating with acarbose, the content of phenylacetylglycine was significantly decreased in the urine of diabetic rats, while the content of other three kinds of endogenous metabolites did not change significantly. This means that phenylacetylglycine related intestinal bacteria metabolic pathway probably was the major pathway during the treatment of acarbose and the effect on other metabolic pathways related to creatinine, hippuric acid, and gluconic acid metabolites was relatively weak.
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