Abstract
Oxidative stress contributes to numerous health issues including neurodegenerative diseases and cancer. Reduced glutathione (GSH) is a major antioxidant that neutralizes reactive oxygen species (ROS) occurring both endogenously through respiration and exogenously during exposure to environmental stressors. Increasing levels of GSH to combat ROS is critical to reducing oxidative stress. The rate‐limiting step of glutathione synthesis is catalyzed by the enzyme glutamate cysteine ligase, a heterodimer consisting of a Glutamate‐Cysteine Ligase Catalytic Subunit (GCLC) and a Glutamate‐Cysteine Ligase Modifier Subunit (GCLM). In this study, we focus on the effect the combination of sulforaphane and maitake mushroom extract has in elevating the production of GSH as a means of preventing cellular damage caused by oxidative stress. HepG2 liver cancer cells were treated over a time course of 24h with maitake mushroom extract (250 μg/mL) and a physiologically achievable level of sulforaphane (0.5 μM). The combination induced gene expression of both GCLC and GCLM an average of 2‐fold as early as 6h. Additionally, we examined the levels of GSH in HepG2 cells treated with the combination.Previously, our laboratory demonstrated that the combination induced the expression of Phase 2 detoxifying enzymes NAD(P)H quinone dehydrogenase 1(NQO‐1), UDP glucuronosyltransferase 1 family, polypeptide A1 (UGT1A1), and heme oxygenase 1 (HMOX‐1/HSP32) as well as reduced the expression of pro‐inflammatory markers interleukin‐6 (IL‐6) and cyclooxygenase‐2 (COX‐2). These results show that the sulforaphane and maitake mushroom extract combination possesses anti‐oxidative properties that may promote cellular health.Support or Funding InformationResearch supported by Nutramax LaboratoriesThis abstract is from the Experimental Biology 2018 Meeting. There is no full text article associated with this abstract published in The FASEB Journal.
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