Abstract
The structures of human telomeric DNA have received much attention due to its significant biological importance. Most studies have focused on G-quadruplex structure formed by short telomeric DNA sequence, but little is known about the structures of long single-stranded telomeric DNAs. Here, we investigated the structure of DNA with a long sequence of d[AGGG(TTAGGG)6] (G6-DNA) and the effect of a single repeat sequence d(TTAGGG) (G01-DNA) on the structure of G6-DNA using sedimentation velocity technique, polyacrylamide gel electrophoresis, circular dichroism spectroscopy, and UV melting experiments. The results suggest that the G6-DNA can form dimers in aqueous solutions and G01-DNA can form additional G-quadruplex structures by binding to G6-DNA. However, G01-DNA has no effect on the structure of DNA with a sequence of d[AGGG(TTAGGG)3] (G3-DNA). Our study provides new insights into the structure polymorphism of long human single-stranded telomeric DNA.
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