Abstract
Streptomyces are bacteria with a very high chromosomal G + C composition (> 70 mol%) and extremely biased codon usage. In order to investigate the relationship between codon usage and gene expression in Streptomyces, we used ssi ( Streptomyces subtilisin inhibitor) as a reporter gene and monitored its secretory expression in S. lividans. In consequence of alteration of the native codons of Leu, Lys and Ser of ssi to minor ones by site-directed mutagenesis, i.e., Leu 79-Leu 80: CTG-CTC to TTA-TTA, Lys 89: AAG to AAA, Ser 108-Ser 109: TCG-AGC to TCT-TCT, respectively, the production of SSI was reduced remarkably in the case of TTA codons, while it was slightly increased in the case of AAA and almost the same in TCT codons. This conspicuous decrease found for Leu codon replacement was probably due to the low availability of intracellular tRNA Leu (UUA), a product of bldA which has been reported to be expressed only during the late stage of growth.
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