Abstract
This study investigated the influence of Canarium album extract (CAext) on intestinal microbiota composition of mice fed a high-fat diet (HFD). Kun Ming (KM) mice were fed either a normal chow diet or a HFD for six weeks. At the seventh week, HFD-fed mice were gavaged daily with saline, or a different dose of CAext for four weeks, respectively. Then, the composition of the gut microbiota was analyzed by high-throughput sequencing technology. Analysis of fecal microbial populations, grouped by phyla, showed significant increases of Firmicutes and Verrucomicrobia, but a decrease of Bacteroidetes in all CAext-fed mice. Particularly, CAext gavage in a low dose or a medium dose caused a significant increase in the proportion of Akkermansia. These findings suggested that CAext can alter the gut microbiota composition of HFD-fed mice, and had a potential prebiotic effects on Akkermansia.
Highlights
The mammalian gut is inhabited by a vast number of microorganisms [1]
To understand the effect of Canarium album extract (CAext) administration on the composition of gut microbiota, the gut microbiota composition was analyzed by Miseq sequencing on the V3–V4 region of 16S rRNA of bacteria
We found that the relative abundance of Verrucomicrobia was statistically found that both the concentration of Firmicutes and Bacteroidetes were significantly increased after a higher in CAext group compared with normal control group (NC) or MC group (Figure 3)
Summary
The mammalian gut is inhabited by a vast number of microorganisms [1]. It has been well recognized that a balanced gut microbiota composition confers benefits to the host, whereas gut microbiota imbalance may lead to various metabolic diseases [2]. As the composition of diet has significant effects on gut microbiota, the interest in food-based strategies able to modulate the gut microbiota composition, and probably their functional effects, has rapidly increased in recent years [3]. It has been reported that plant-derived polysaccharide foods increase colonic bifidobacterial numbers [4], whereas a long-term diet rich in saturated fat, such as high-fat diet (HFD), increases the proportion of Firmicutes to Bacteroidetes, which is associated with obesity-induced metabolic diseases [5,6,7]. Polyphenols can be converted to bioactive compounds that may exert physiological effects by the gut microbiota, while the composition of microbiota can be modified by polyphenols [10]
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