Abstract

Objective To investigate the effect of recipient derived dendritic ceils (DC) loaded with PUVA-treated donor splenic lymphocytes (PUVA-SP) on humoral and cellular immunity and allograft rejection in allograft recipients. Methods Heterotopic vascularized heart transplantation model was established from inbred DA to LEW rat. DA rat spleen lyrnphoeytes were treated with 8-methoxypsoralen plus UVA irradiation (PUVA). LEW rat bone marrow-derived DC were co-cultured with PUVA-treated or untreated DA spleen lymphocytes (PUVA-SP or SP), and the costimulatory molecules (CD86 and CD80) of the treated DC (PUVA-SP-DC or SP-DC) and untreated DC were detected by flow cytometry. Recipient LEW rats were subjected to infusion of PUVA-SP-DC, SP-DC or PBS through the peripheral vein at the 7th day before heart transplantation. Cardiac allgrafts survival was evaluated by daily palpation. Circulating alloantibody IgG levels of recipient rats were determined by flow cytometry, and proliferative responses of recipient T cells to donor or third party antigen were examined by mixed leukocyte reaction (MLR). The spleen was collected at the 6th day after transplantation. Results PUVA treatment effectively induced apoptosis of DA rat spleen lymphocytes. After co-cultured with donor PUVA-SP, recipient DC still maintained an immature phenotype with low levels of CD80 and CD86. Injection of PUVA-SP-DC significantly decreased serum anti-donor alloantibody levels of recipient rats, induced donor antigen specific hyporesponsiveness of recipient T cells,and prolonged allograft survival. Moreover, the spleens of the recipient rats, infused with PUVA-SP-DC,were the smallest compared to those of the recipients infused with PBS or SP-DC.Conclusion PUVA-SP-DC could effectively down-regulate anti-donor cellular and humorat adaptive immunity, leading to prolongation of cardiac allograft survival Key words: Dendritic cells; Heart transplantation; Antibody formation; Immunity,cellular

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