Abstract
<i>Background</i>: Effective treatment against non-alcoholic fatty liver disease (NAFLD) is lacking. Song Zhi Wan (SZW), a Chinese formulation medicine comprising eight herbal ingredients, has been demonstrated to confer a liver protective effect in chronic hepatitis C patients. We aimed to investigate the effect of SZW on NAFLD using a high-fat diet (HFD; 60% fat) induced obese mouse model. <i>Methods</i>: C57BL/6J mice were fed with HFD for 10 weeks, followed by daily oral administration of various dosages of SZW (low [n=6], normal [n=10], high [n=10]) or water (n=10) for 8 weeks. Another formulation of SZW (modified SZW), in which two ingredients were replaced by radish seed and barley, was tested. Serum total cholesterol and triglyceride levels, liver transaminases, and histologic steatosis were assessed. <i>Results</i>: At the end of experiment, the HFD-fed placebo mice had a mean increase in serum total cholesterol and triglyceride by 57.7% and 35.0%, respectively. HFD-fed mice receiving either SZW formulation had a smaller increase in serum total cholesterol (mean increase 7.9% – 39.4%) and a significant reduction in triglyceride (mean reduction 4.2% – 27.4%; <i>P</i> < 0.05). A dosage dependent effect on serum total cholesterol and triglyceride was observed with modified SZW (=0.043 and 0.006, respectively). 90% of placebo mice and 59% of SZW-treated mice had severe steatosis (<i>P</i>=0.079). With an escalating dosage of original SZW, there was a decreasing proportion of mice with severe steatosis (<i>P</i>=0.013). <i>Conclusion</i>: SZW administration can attenuate hypercholesterolemia, reduce dyslipidemia and alleviate steatosis in HFD-induced obese mice.
Highlights
Nonalcoholic fatty liver disease (NAFLD) is defined by excessive fat accumulation in the liver that is not a result of alcohol and other definite hepatotoxins
This study aimed to evaluate the efficacy of Song Zhi Wan (SZW) in reducing steatosis, alleviating liver damage, and regulating dyslipidemia in this high-fat diet (HFD) induced non-alcoholic fatty liver disease (NAFLD) mouse model
At week 0 of intervention, mice fed with HFD were significantly heavier than those fed with low-fat control diet (LFD)
Summary
Nonalcoholic fatty liver disease (NAFLD) is defined by excessive fat accumulation in the liver that is not a result of alcohol and other definite hepatotoxins. NAFLD is a common liver disease with increasing global health concern, with an estimated global prevalence of 25.2% [1]. Methods: C57BL/6J mice were fed with HFD for 10 weeks, followed by daily oral administration of various dosages of SZW (low [n=6], normal [n=10], high [n=10]) or water (n=10) for 8 weeks. Another formulation of SZW (modified SZW), in which two ingredients were replaced by radish seed and barley, was tested. Results: At the end of experiment, the HFD-fed placebo mice had a mean increase in serum total cholesterol and triglyceride by 57.7% and 35.0%, respectively. Conclusion: SZW administration can attenuate hypercholesterolemia, reduce dyslipidemia and alleviate steatosis in HFD-induced obese mice
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