Abstract
Gravid skipper frogs were treated with ICI 33,828, a nonsteroidal pituitary inhibitor, for 8 days. The response of pituitary-induced spawning in the treated frogs showed a considerable reduction when compared to that of the controls. Further, the spawned eggs of the treated animals lacked the jelly coat. While there was an increase in the ovarian cholesterol concentration in the treated animals, no significant change in the ovarian ascorbic acid level was noticed. The possible hormonal mechanism involved is discussed.
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