Abstract
To prevent the breaking of the culture dish from the impact of bubble growth around a pressure source, we experimentally investigated the effect of doubly connected cylindrical waveguides with different diameters on extracting high-intensity pressure pulses generated by an underwater spark discharge with pulse energy less than 0.5 J. The experiments were conducted using water-filled cylindrical holes 4 mm and 14 mm in diameter. The proposed structure was fixed at a position 2 mm away from the discharge electrode. When the length of the 4 mm diameter hole was 4 mm, it was revealed that an intense pressure pulse exceeding 20 MPa in peak pressure without breaking the dish bottom was obtained even at a position 13 mm away from the discharge electrode. Based on this result, we also observed adherent cells seeded on a glass-bottom culture dish before and after irradiating an intense pressure pulse.
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