Abstract
The content of reactive oxygen species, the activity of key antioxidant enzymes ‒ ascorbate peroxidase and glutathione reductase, the level of expression of the hypersensitive response marker gene (HSR) as well as potato virus X particles content in cv. Uladar seedlings grown on an ion-exchange substrate in the presence of a complex preparation containing chitosan, Bacillus subtilis bacteria-based preparation and salicylic acid, when infected potato virus X. Accumulation of the reactive oxygen species, increase in ascorbate peroxidase activity, a lower level of HSR gene expression and a lower content of virus X particles in potato plants under such conditions are shown. An increase in both the number of potato minitubers and dry matter content in them was also registered when plants were grown on an ion-exchange substrate in the presence of a complex preparation.
Highlights
well as potato virus X particles content in cv
Uladar seedlings grown on an ion-exchange substrate in the presence
when plants were grown on an ion-exchange substrate in the presence
Summary
The content of reactive oxygen species, the activity of key antioxidant enzymes ‒ ascorbate peroxidase and glutathione reductase, the level of expression of the hypersensitive response marker gene (HSR) as well as potato virus X particles content in cv. Пробы листьев растений для изучения содержания АФК, активности антиоксидантных ферментов – АПР и ГР, а также экспрессии гена маркера гиперчувствительного ответа (HSR) отбирали на 20-е сутки (за 10 сут до заражения) и на стадии бутонизации на 50-е сутки (спустя 20 сут после заражения ХВК) [8,9,10].
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