Abstract
The aim of the study was to determine the effect of a combination of medium chain triglycerides (caprylic, capric, caproic and lauric acids), linoleic acid (essential fatty acid), vitamins A and E and soy lecithin, through a morphometric study, on the wound healing kinetics of experimental cutaneous ulcers. A total of 45 male Wistar rats were used, in which a skin flap of total thickness with an area of 4 cm2 was removed. The animals were divided randomly into 3 groups of 15 rats each, Control, Reference and Test groups, which were treated topically with 0.9% NaCl, a preparation of clostebol combined with neomycin sulfate and the test formulation, respectively. The wound areas were measured by digital planimetry at days zero, 3, 7 and 12 postoperative. Based on the wound area, we determined the degree of tissue repair and mean rate of repair at different time intervals. At day 3, an expansion of the wound area was observed in the Reference group and slight contraction in the Control and Test groups. On the subsequent days, the healing process, according to the degree of repair, proceeded in a linear manner, such that, at day 12, the healed area reached 77.95% of the initial ulcerated region in the Control group, 78.40% in the Reference group and 83.49% in the Test group, showing no significant differences. The overall mean rate of repair was equally similar at 12 days of treatment: 25.79 mm2/dia in the Control group, 25.42 mm2/dia in the Reference group and 27.38 mm2/dia in the Test group. The test preparation, applied topically on the experimentally induced cutaneous ulcers in rats, did not accelerate the process of tissue repair by secondary union.
Highlights
The phenomenon of wound healing permits surgical interventions to be performed in animals
The aim of this work was to determine the effect of the combination of medium chain triglycerides, linoleic acid, vitamins A and E and soy lecithin, on the tissue repair kinetics of experimental cutaneous ulcers, by means of a morphometric study
The degree of wound repair (DR) of the ulcers was determined on days 3, 7 and 12, where it was calculated based on the initial area and expressed as a percentage, according to the following formula[15,16]
Summary
The phenomenon of wound healing permits surgical interventions to be performed in animals. Humans observed that wounds sustained from injuries closed themselves after a period of time. Hippocrates conducted studies on wounds and noted that tissues have the power of natural wound healing. Some studies were conducted on wound healing, but based on empirical observations in battle injuries. In the beginning of the XXth century, studies on laboratory animals were begun, comparing wounds in humans. Experimentations intensified and permitted a greater understanding of this subject[1]
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