Effect of a chemical dispersant (Corexit 9500A) on the structure and ion transport function of blue crab (Callinectes sapidus) gills

Abstract

Chemical dispersants are commercially available mixtures of surfactants and solvents that have become important tools in the remediation of spilled oil. Given the importance of oil to the world economy, the recurring nature of spills, and the prevalence of dispersant use in remediation, there is a critical need to understand potential toxic impacts of dispersants on invertebrate and vertebrate animals. Blue crabs (Callinectes sapidus) play ecologically important roles in the environments they inhabit and support economically important fisheries along the Atlantic Coast and in the Gulf of Mexico. In studies reported here, we assessed the impact of a chemical dispersant, Corexit 9500A, on the structure and ion transport function of blue crab gills. Exposure of blue crabs to Corexit 9500A for 24 h (0–300 ppm in artificial seawater under static conditions) revealed a 24-h lethal concentration 50 (LC50) estimate of 210 ppm. A histological analysis of gills from crabs exposed for 24 h to a sub-lethal concentration of Corexit 9500A (125 ppm) revealed evidence of loss or disruption of cuticle, and an increase in stained amorphous material in the hemolymph spaces of gill lamellae. Quantitative image analysis of stained gill sections revealed the area/length ratio of gill lamellae in crabs exposed to Corexit 9500A (24 h, 125 ppm), was greater than that in gill lamellae from control crabs; the results are consistent with the presence of edematous swelling in gill lamellae from dispersant-exposed crabs. Quantitative PCR was used to measure the relative abundance of transcripts encoding three ion transport proteins (Na+/K+ ATPase, plasma membrane Ca2+ ATPase (PMCA), and sarcoplasmic reticulum/endoplasmic reticulum Ca2+ ATPase (SERCA)) in gills from Corexit-exposed and control crabs. In general, the abundance of transcripts encoding each ion transport protein was lower in gills from dispersant-exposed crabs than in gills from control crabs. The combined results are consistent with the hypothesis that 24-h exposure of blue crabs to a sublethal concentration of Corexit 9500A impacts both the structure and ion transport function of gills.