Abstract
Effect of 8-anilino-1-naphthalene sulfonic acid (ANS) on the interaction between thyroid hormone and anti-thyroid hormone antibodies was examined. Addition of 5.1 × 10 6 or 6.3 × 10 6 molar excess of ANS, respectively, enhanced the binding of 125I-T 3 or 125I-T 4 to their respective autoantibodies. However, further increase in ANS concentration resulted in a decrease in binding. These results suggest that the optimum concentration of ANS for the detection of anti-thyroid hormone antibodies is 5 ~ 6 × 10 6 molar excess of ANS to 125I-T 3 or 125I-T 4. On the other hand, addition of 10 4 molar excess of ANS concentration decreased the binding of 125I-insulin to anti-insulin antibodies. It was therefore suggested that the effect of ANS could be a nonspecific one and likely due to its negative charge.
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