Abstract
To investigate the influence of 650 nm GaAlAs laser on the action of bone morphogenetic protein (BMP) in bone defects produced in rat femurs. The sample consisted of 12 male albino Wistar rats (Rattus norvegicus). The animals were randomly divided into four experimental groups. After undergoing anesthesia, the fur was removed from the lateral face of the right thigh and surgical dissection was performed to view the femur region. A bone defect was created using a spherical diamond-tipped drill bit. In groups 1 and 2, the defect was filled with a paste of Gen-Tech bone-inducing substance. The animals were treated with GaAlAs laser, at a predetermined dose of joules/cm(2) for 80 seconds, over an area of 1 cm(2). Groups 2 and 4 were used as controls. Bone samples were removed to perform histological procedures and morphometric analyses on the 7th, 14th and 21st days after the operation. The results obtained were subjected to statistical analysis using ANOVA variance according to two criteria, with four repetitions, followed by the post hoc t test. The rejection level for the nullity hypothesis was 0.05 or 5% (alpha < or = 0.05). In comparisons between G1, G2, G3 and G4, p = 0.024 was observed. In statistical comparisons using the t test for paired samples, only G1 vs. G4 presented a statistically significant result (p = 0.021). The association of low-power laser application and Gen-Tech bone-inducing substance achieved a better result than laser application alone or BMP use alone.
Highlights
Experimental groupsIt has been estimated that 5.6 million bone fractures occur in the United States every year, of which 5% to 10% evolve to delayed consolidation or pseudoarthrosis
From the general means for the neoformed bone tissue in the groups, comparisons were made between G1, G2, G3 and G4 by means of analysis of variance (ANOVA) which gave the result of p = 0.024
We utilized Wistar rats as the experimental model, considering the positive bone tissue response characteristics in this mammal, which resemble those found in humans, and the ease of acquiring and handling these animals. 7,8,10,11 When the animals in this study were analyzed on the seventh day after creating the bone defects, it was observed that Group 1 was at a more advanced stage of repair than the other groups studied, presenting an area of vessel neoformation, neoformed bone tissue with a large concentration of osteoblasts and absence of inflammatory reaction
Summary
Experimental groupsIt has been estimated that 5.6 million bone fractures occur in the United States every year, of which 5% to 10% evolve to delayed consolidation or pseudoarthrosis. There are studies in the field of molecular biology, concerning the discovery of new biomaterials and the elucidation of repair components, with regard to the action of growth factors and their capacity to promote the differentiation of immature osteoprogenitor mesenchymal cells into osteocompetent cells These have greatly contributed towards consolidating the results relating to techniques utilized in bone repairs[3,4]. Among the functions of the carrier are to delay the rapid dispersion of the BMP from the implantation site and protect against non-specific lysis[6] Within this context, with the evolution of biotechnology and medical sciences, low-power lasers have become popular. The purpose of the present study is to investigate the action of low-power laser on BMPs for repairing bone defects
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