Abstract
Immunosuppressive potential of 65 kDa buffalo placental protein (bPP65) on B-cell proliferation in vitro and antibody response in vivo was evaluated. B-cell proliferation was estimated by measuring incorporation of tritiated thymidine in buffalo lymphocytes while primary antibody responses against phytohaemagglutinin (PHA) or keyhole limpet haemocyanin (KLH) were evaluated in mice. bPP65 suppressed proliferation of lipopolysaccharide (a B-cell specific mitogen)-stimulated buffalo lymphocytes in vitro indicating suppression of B-cells. This suppression was dose dependent over the protein concentration range 25-100 µg/ml. Primary antibody responses in mice against PHA and KLH in presence of bPP65 were lower as compared to in its absence but these were not statistically significant. Amino acid composition data of bPP65 and BSA suggested that bPP65 is different from BSA. (Asian- Aust. J. Anim. Sci. 2002. Vol 15, No. 3 : 432-437)
Highlights
The placenta of mammals is an active endocrine organ mediated through T-cells, contribution of suppressed secreting numerous proteins, prostaglandins and steroids. primary antibody response can not be ignored
B-cell proliferation was estimated by measuring incorporation of tritiated thymidine in buffalo lymphocytes while primary antibody responses against phytohaemagglutinin (PHA) or keyhole limpet haemocyanin (KLH) were evaluated in mice. bPP65 suppressed proliferation of lipopolysaccharide-stimulated buffalo lymphocytes in vitro indicating suppression of B-cells
Having immunomodulatory action at fetomaternal interface cells and in the present study, the effect of 65 kDa buffalo to contribute to non-rejection of the fetus inheriting genes placental protein on B-cell proliferation in vitro from father as well as mother
Summary
The placenta of mammals is an active endocrine organ mediated through T-cells, contribution of suppressed secreting numerous proteins, prostaglandins and steroids. primary antibody response can not be ignored. ABSTRACT :Immunosuppressive potential of 65 kDa buffalo placental protein (bPP65) on B-cell proliferation in vitro and antibody response in vivo was evaluated. B-cell proliferation was estimated by measuring incorporation of tritiated thymidine in buffalo lymphocytes while primary antibody responses against phytohaemagglutinin (PHA) or keyhole limpet haemocyanin (KLH) were evaluated in mice.
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