Effect of 6-BA on the Plant Regeneration via Organogenesis from Cotyledonary Node of Cowpea (Vigna unguiculata L. Walp)

Manman Tie,Qian Luo,Huanxiu Li,Yanxia Zhu

doi:10.5539/jas.v5n5p1

Abstract

The present study compares effects of different concentrations of 6-BA on regeneration from cotyledonary node explants of cowpea (Vigna unguiculata L. Walp). The seeds were inoculated on MSB5 medium [Murashige and Skoog (1962) salts and Gamborg B5 vitamins (1968)] containing different concentrations (0, 1, 2, 3, 4, 5 mg/L) of 6-BA for 4 days. The cotyledonary node explants with one cotyledon excised from 4-day-old seedlings, placed in vitro on MSB5 medium containing 6-BA at different dose (0, 0.5, 1.0, 1.5 mg/L) for shoot induction and elongation. Best response in terms of shoot number and shoot length were obtained with explants derived from seedling preconditioning with 3mg/L 6-BA followed by the induction and elongation stage pretreated with 0.5mg/L 6-BA. The elongated shoots were rooted on MSB5 medium without hormone.

Highlights

Cowpea (Vigna unguiculata L.) is widely grown in Africa, Latin America, Southeast Asia and southwestern regions of North America, and is a major source of high-quality dietary protein and energy for local people
The cotyledonary node explants with one cotyledon excised from 4-day-old seedlings, placed in vitro on MSB5 medium containing 6-BA at different dose (0, 0.5, 1.0, 1.5 mg/L) for shoot induction and elongation
Limited genetic diversity in cowpea breeding programs is of special concern because cowpea appears to have lower inherent genetic diversity than other cultivated crops as a result of a hypothesized single domestication event (Fang et al, 2007)

Summary

Introduction

Cowpea (Vigna unguiculata L.) is widely grown in Africa, Latin America, Southeast Asia and southwestern regions of North America, and is a major source of high-quality dietary protein and energy for local people. It plays an important role in the lives of millions of people in developing countries of Africa and Asia. An effective and rapid regeneration protocol is essential for genetic transformation. The aim of this paper is to explore the effect of 6-BA on different stages of regeneration of cowpea, to provide a theoretical and technical basis for rapid propagation

Plant Materials and Seeds Preconditioning

Data Collection and Statistical Analysis

Findings

Effect of 6-BA Preconditioning on Seed Germinating