Abstract
1. A mitotically synchronized population of HeLa cells was exposed to 5-bromodeoxyuridine for 3-h intervals spaced over the entire division cycle. The maximum loss in cell viability took place when the exposure to 5-bromodeoxyuridine occurred during the interval when the rate of DNA synthesis was a maximum. 2. Following a 24-h exposure to the analog, DNA synthesis was inhibited in the subsequent replication cycle whereas RNA and protein synthesis was slightly reduced. 3. The activity of DNA polymerase (deoxynucleoside-triphosphate:DNA deoxynucleotidyltransferase, EC 2.7.7.7) and uridine kinase (ATP:uridine 5′phosphotransferase) was reduced by a significantly large percentage relative to changes in total cellular protein. This may imply alteration in functional protein consequent to the replacement of DNA thymine by 5-bromouracil.
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Schedule a callMore From: Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis
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