Abstract
The purpose of this study was to investigate the effect of T 3 on amino acid accumulation and on the membrane potential of Sertoli cells of immature rat testes. Testes of pre-pubertal and pubertal rats were pre-incubated (30 min) in Krebs-Ringer bicarbonate buffer and incubated in the presence of [ 14C]methylaminoisobutyric acid with and without T 3 for 15, 45 and 60 min. The hormone (10 −6 M and 10 −7M) significantly stimulated amino acid accumulation in 6 and 13-day old rat testes but did not have any effect in neonatal and pubertal animals. T 3 produced a dose-dependent hyperpolarizing effect at concentrations of 10 −6 M, 10 −5 M, 2 × 10 −5 M and 10 −4 M. We conclude that T 3 induces a membrane hyperpolarization in Sertoli cells and stimulates amino acid accumulation in immature rat testes, demonstrating that the hormone has a rapid plasma membrane action.
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