Abstract
An early life-stage (ELS) test was conducted with the zebrafish and 3,4-dichloroaniline (3,4-DCA) in eight laboratories. Based on the results from all eight laboratories, an LOEC of 200 μg 3,4-dichloroaniline/liter applies for the early life stages of the zebrafish. Effects observed were reduction of the survival rate and malformations. If the 100 μg/liter concentration additionally tested by one laboratory is included in the assessment, an LOEC of 100 μg 3,4-DCA/liter is obtained for the survival rate and increase in length. The NOEC is 20 μg/liter. The present results of a comparative laboratory study with the zebrafish show that a 42-day ELS test can be conducted with this species of fish, and affords meaningful results.
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