Effect of 2-Phenoxyethanol and Chilling on the Hemolymphatic Parameters of the Small Abalone, Haliotis diversicolor

Abstract

Collecting hemolymph is essential for the physiological study of the small abalone, Haliotis diversicolor. Herein, we used two methods of anesthesia, 2-phenoxyethanol (2-PE) and chilling, to allow easy handling of the abalone and investigated their influence on hemolymphatic parameters. 2-PE has been proven effective for exfoliating abalones from the attached substratum and reduces injuries caused by mechanical removal. Chilling can reduce the mobility of abalone. The results indicated that the both methods were stressors which caused changes in some parameters such as the number of hemocytes and concentrations of glucose, protein, and hemocyanin, in the abalone. Therefore, direct removal and quick bleeding of experimental abalones may be a better method to collect hemolymph from abalone.